畜牧兽医学报 ›› 2015, Vol. 46 ›› Issue (4): 568-575.doi: 10.11843/j.issn.0366-6964.2015.04.009

• 遗传繁育 • 上一篇    下一篇

表达谱芯片筛选獭兔毛色相关候选差异表达基因

施丽娟1,2,秦立志1,2,王文洲1,2,闫晓荣1,2,翁巧琴3,吴信生1,2*   

  1. (1.扬州大学动物科学与技术学院,扬州 225009;2.江苏省动物繁育与分子设计重点实验室,扬州 225009;3.浙江省余姚市欣农兔业有限公司,余姚 315400)
  • 收稿日期:2014-09-05 出版日期:2015-04-23 发布日期:2015-04-23
  • 通讯作者: 吴信生,教授,博士,E-mail:xswu@yzu.edu.cn
  • 作者简介:施丽娟(1990-),女,江苏南通人,硕士生,主要从事动物遗传育种与繁殖研究,E-mail:msbdmy@126.com
  • 基金资助:

    现代农业产业技术体系建设专项资金(CARS-44-1);江苏省高校优势学科建设工程资助项目(PAPD-2014-134)

Screening Candidate Differentially Expressed Genes of Rex Rabbits’ Fur Color with Gene Chips

SHI Li-juan1,2,QIN Li-zhi1,2,WANG Wen-zhou1,2,YAN Xiao-rong1,2,WENG Qiao-qing3,WU Xin-sheng1,2*   

  1. (1.College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China;2.Jiangsu Key Laboratory of Animal Genetics & Breeding and Molecular Design,Yangzhou 225009,China;3.Zhejiang Yuyao Xinnong Rabbit Industry Co.,Ltd.,Yuyao 315400,China)
  • Received:2014-09-05 Online:2015-04-23 Published:2015-04-23

摘要:

本研究旨在了解青紫蓝色獭兔和白色獭兔毛色形成的分子遗传学机理。试验分为3组,每组包含全同胞青紫蓝獭兔和白色獭兔各1只。利用安捷伦基因芯片技术分析不同分组之间差异基因表达情况,并通过实时荧光定量PCR方法对部分差异显著基因进行验证。差异表达分析结果显示,3组青紫蓝色獭兔对白色獭兔分别筛选出545、1 680和2 092个差异表达基因 (Differentially expressed genes,DEGs),共有172个共同基因;GO(Gene Ontology)分类结果显示,这些基因主要参与细胞分化、增殖、凋亡、发育、离子转运、免疫应答等生物学过程。通过实时荧光定量PCR技术对Creb1、Wnt2、GnaqGna14基因在组织样中的表达量进行检测,结果与表达谱芯片一致。通过表达谱芯片结果结合生物信息学分析和文献报道,筛选出了Creb1、THWnt2、GnaqDvl1、Mapk12等对毛色形成可能具有重要影响的候选基因。

Abstract:

In order to explore the molecular genetic mechanism of fur color formation between chinchilla rex rabbits and white rex rabbits.The experiments were divided into 3 groups,every group contained one chinchilla rex rabbit and one white rex rabbit,which came from full-sib family.The Agilent’s rabbit gene expression microarray was used to determine the differentially expression genes between different fur color groups of rex rabbits,and the expression patterns of selected differentially expressed genes were further identified by quantitative real-time PCR.The results showed that 545,1 680 and 2 092 differentially expressed genes associated with fur color of rex rabbits were screened out from 3 groups of chinchilla rex rabbits and white rex rabbits,respectively.A total of 172 mutual genes were found among these 3 rex rabbits groups.GO (Gene Ontology) analysis results revealed that the differentially expressed genes were related to cell differentiation,proliferation,apoptosis,growth,ionic transport altered and immune response,etc.The quantitative real-time PCR showed gene expression trend of Creb1,Wnt2,Gnaq and Gna14 genes was consistent with that of gene expression microarray data,indicating that the result of gene chip was reliable.Combined with bioinformatic analysis and related literatures,some genes were potentially related to fur color formation,such as Creb1,THWnt2,GnaqDvl1,Mapk12,and so on.

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